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lactobacillus
Posted: 17 May 2007, 10:09
by Terry
Hi All
Does anyone know how to cultivate lactobacillus from yoghurt on plates - which medium do you use?
Thanks
Posted: 17 May 2007, 10:53
by Ocean Breeze
Use tomato juice agar. It works really well.
Posted: 17 May 2007, 11:14
by Ocean Breeze
Sorry... forgot to post link
http://www.bd.com/ds/technicalCenter/in ... _Media.pdf
This isnt the one that I make, but cant seem to lay my hands on my recipe at the moment.
Lactobacillus
Posted: 17 May 2007, 13:41
by rae
Hi Rainbow-sky,
I'm very interested in getting the tomato juice agar recipe but I can't seem to get into the web site.
If you find your copy could you fax it to me.
Attention: Lorrae
Fax No: 02 48613328
Thanks in anticipation
Lorrae
Posted: 18 May 2007, 13:12
by Ocean Breeze
Hi ... havent had time to search for my recipe... heres a cut & paste of anohter one... it should be similar. all the best.
Formulae
Difcoâ„¢ Tomato Juice Agar
Approximate Formula* Per Liter
Tomato Juice (from 400 mL) ..................................... 20.0 g
Peptone ................................................................... 10.0 g
Peptonized Milk ....................................................... 10.0 g
Agar ......................................................................... 11.0 g
Difcoâ„¢ Tomato Juice Agar Special
Approximate Formula* Per Liter
Tomato Juice (from 400 mL) ..................................... 20.0 g
Peptone ................................................................... 10.0 g
Peptonized Milk ....................................................... 10.0 g
Agar ......................................................................... 20.0 g
Difcoâ„¢ Tomato Juice Broth
Approximate Formula* Per Liter
Tomato Juice (from 400 mL) ..................................... 20.0 g
Yeast Extract ............................................................ 10.0 g
Dextrose ................................................................... 10.0 g
Dipotassium Phosphate .............................................. 0.5 g
Monopotassium Phosphate ........................................ 0.5 g
Magnesium Sulfate .................................................... 0.1 g
Sodium Chloride ........................................................ 0.01 g
Ferrous Sulfate ........................................................... 0.01 g
Manganese Sulfate .................................................... 0.01 g
*Adjusted and