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Re: Pasteur experiment glass tubing

Posted: 21 May 2009, 19:48
by Labbie
That just awful, should be a bottle of wine, flowers, chocolates and a week off work. 8O 8O

Re: Pasteur experiment glass tubing

Posted: 22 May 2009, 10:39
by smiley
Phenolphthalein in the coffee - just a drop, no need to go overboard! :thumbup:

Re: Pasteur experiment glass tubing

Posted: 22 May 2009, 10:55
by bindi
Help! Just finish this experiment and again it didnt work :crazy:
I use the plastic airlocks but seem always to grow mold when Im not suppose too :?
We sterilise using Miltons, broth is boiled for 10min but seem to still grow something!! does anyone have any FOOL (which is obviously me :-? ) proof method?

Re: Pasteur experiment glass tubing

Posted: 22 May 2009, 11:32
by ELIZABETH
Hi All,
This one works every time for us.
Liz

http://www.chemheritage.org/Educational ... spapas.htm

Re: Pasteur experiment glass tubing

Posted: 22 May 2009, 11:43
by bindi
Thanks Liz, but we dont have an autoclave, so we sterilise then boil. Anyone out there do similar with good results?

Re: Pasteur experiment glass tubing

Posted: 22 May 2009, 13:04
by lada
We boil the broth for 10min. One flask has a stopper with thistle funnel through it ( see "why" in a minute) and another with stopper with the funky air lock tube from brewery. This airlock has a wide mouth on top, hence thistle funnel for control. We had 4 each and every one with funnel grew mould and crap (it was left for too long) and the airlock ones were clear. Very happy with results :clap3: :clap3:
lada

Re: Pasteur experiment glass tubing

Posted: 22 May 2009, 14:17
by bindi
After questioning our Mr B :cheesy: found out he is not boiling with the air locks and straight tubes on, this could be our problem!
thanks Lada!