Aceto Orcein Stain alternative

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Lis
Posts: 362
Joined: 01 Jun 2006, 10:00
Job Title: Laboratory Technician
School: Central Coast Grammar
Suburb: Erina Heights
State/Location: NSW

Aceto Orcein Stain alternative

Post by Lis »

Hi All

I need to have aceto orcein stain, by Friday and of course I dont have any, something else for my order list, does anyone know of an alternative stain for observing chromosomes and cell division, any help would be greatly appreciated

thanks Lisa
ellice
Posts: 193
Joined: 27 Jul 2007, 15:29
Job Title: Lab technician
School: The Illawarra Grammar School
Suburb: Figtree
State/Location: NSW

Re: Aceto Orcein Stain alternative

Post by ellice »

Hi Lisa,
We used to do this prac, but never had much success. So we purchased prepared slides.
Sorry I can't help with an alternative stain (I don't think there is one).
Ellice. 8-)
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Sassi
Posts: 432
Joined: 17 Sep 2007, 12:43
State/Location: NSW

Re: Aceto Orcein Stain alternative

Post by Sassi »

There may be a few options, depending upon which type of cells to stain??? I did a quick search on google for 'stains for chromosomes' and there seems to be maybe something you can use...

Good luck :thumbup:
novicegirl
Posts: 7
Joined: 12 Mar 2009, 10:24
State/Location: VIC

Re: Aceto Orcein Stain alternative

Post by novicegirl »

Try ringing a histopathology department, either in a hospital or private lab. They will have recipes for staining cells in division.
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