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Pepsin prac and changing pH
Posted: 28 Oct 2009, 10:22
by rae
Please Help!!
We are about to do the enzyme prac with pepsin and egg whiteand changing the pH.
Can any one suggest easy solution to use to achieve pH 2, pH6/7 and pH9? What molarities??
I was thinking acetic acid and sodium bicarb am I on the right track?
Lorrae
Re: Rennin prac and changing pH
Posted: 28 Oct 2009, 10:38
by Whspa
I've had success with this:
pH2 0.01M hydrochloric acid
pH3 80mL 0.1M citric acid plus 20mL 0.2M disodium hydrogen phosphate
pH4 62mL 0.1M citric acid plus 38mL 0.2M disodium hydrogen phosphate
pH5 48mL 0.1M citric acid plus 52mL 0.2M disodium hydrogen phosphate
pH6 37mL 0.1M citric acid plus 63mL 0.2M disodium hydrogen phosphate pH7 18mL 0.1M citric acid plus 82mL 0.2M disodium hydrogen phosphate
pH8 3mL 0.1M citric acid plus 97mL 0.2M disodium hydrogen phosphate
pH10 0.1M sodium hydroxide
These solutions are sufficiently accurate for use in the cell membrane activity in Biology and also for general pH testing
Carol
Re: Pepsin prac and changing pH
Posted: 28 Oct 2009, 11:26
by rae
Does this mean, if I add these buffers to a pepsin solution they will end up being the pH of the buffer??
Re: Pepsin prac and changing pH
Posted: 29 Oct 2009, 08:16
by Whspa
I add the pepsin directly to the buffers.
Carol
Re: Pepsin prac and changing pH
Posted: 30 Oct 2009, 09:42
by Mother
I'll come in on this one Carol.
Pepsin powder or a pepsin solution?
Re: Pepsin prac and changing pH
Posted: 30 Oct 2009, 10:19
by Whspa
Pepsin powder