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How do I make up INDIGO CARMINE stain?
Posted: 24 Jan 2013, 14:01
by Ian
Hi all, and I hope you are all having a good start to the New Year.
I need to make up some Indigo Carmine microscopy stain, as my supply is getting low. I have a little bottle of powder, but my "Bible" ("The Laboratory", by Dungey) does not seem to have a recipe for making it up. Can anybody help me out here?
cheers
Ian
Re: How do I make up INDIGO CARMINE stain?
Posted: 25 Jan 2013, 07:50
by dss8386
I found thison the net. this is the website
http://labnetwest.asn.au/resources/technical-tips/
Indigo Carmine recipes:
1. 0.25g indigo carmine in 100mL of 50% ethanol.
pH range 11.6 to 13.0 , colour change blue to yellow.
2. 1.0g per litre of 50% ethanol pH 11.6-14, blue to yellow.
Re: How do I make up INDIGO CARMINE stain?
Posted: 25 Jan 2013, 12:25
by Ian
Thanks Dave. I'll give that a go. (It is more information than what I had!)
Is there a difference between Indigo Carmine used as a Microscopy stain and Indigo Carmine used as a pH indicator?
Thanks again
ian
Re: How do I make up INDIGO CARMINE stain?
Posted: 25 Jan 2013, 12:47
by dss8386
I cant find any information but I found this document on making up solutions etc. Really useful and does have a recipe for indigo carmine
http://inside.bard.edu/~bjude/resources ... uide-1.pdf
Re: How do I make up INDIGO CARMINE stain?
Posted: 30 Jan 2013, 09:42
by mtg
I have a recipe for the indicator not sure if this will work as a stain. According to my lab manual (which I will be buried with) : Weight of indicator and volume of ethanol for 1 litre: 1.0 g to 500ml ethanol up to 1 litre with water. (pH range 11.6 -14, colour change blue-yellow) Hope this helps you. My source is A Handbook of Laboratory Solutions. M.H. Gabb and W.E.Latchem 1967. Edited by Philip Kogan and published by ANDRE DEUTSCH limited. Just checked with biol teacher and he reckons it should work.