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DNA stains
Posted: 26 Aug 2013, 10:52
by suea
Does anyone know what to stain DNA with and where to get it. After extraction, it would be great to look at it!
Re: DNA stains
Posted: 26 Aug 2013, 11:33
by lada
We do not stain it . Students can see the strands in the t/tube or they can pull it out with tweezers.
Lada
Re: DNA stains
Posted: 26 Aug 2013, 11:36
by Graham Kemp
suea wrote:Does anyone know what to stain DNA with and where to get it. After extraction, it would be great to look at it!
Any of the usual cell dyes will work.
Toluidine Blue,
Methylene Blue, or
Aceto-Orcein will all stain the nucleus.
Methylene Blue seems to work quite well, and is commonly suggested in text books.
If this is for the DNA extraction from
dried peas, our biology teacher always recommends using
bananna or
kiwi fruit as substitutes for the peas to give better results.