lactobacillus

[Terry]

Hi All
Does anyone know how to cultivate lactobacillus from yoghurt on plates - which medium do you use?
Thanks

[Ocean Breeze]

Use tomato juice agar. It works really well.

[Ocean Breeze]

Sorry... forgot to post link

http://www.bd.com/ds/technicalCenter/in ... _Media.pdf

This isnt the one that I make, but cant seem to lay my hands on my recipe at the moment.

[rae]

Hi Rainbow-sky,

I'm very interested in getting the tomato juice agar recipe but I can't seem to get into the web site.
If you find your copy could you fax it to me.
Attention: Lorrae
Fax No: 02 48613328

Thanks in anticipation
Lorrae

[Ocean Breeze]

Hi ... havent had time to search for my recipe... heres a cut & paste of anohter one... it should be similar. all the best.

Formulae
Difcoâ„¢ Tomato Juice Agar
Approximate Formula* Per Liter
Tomato Juice (from 400 mL) ..................................... 20.0 g
Peptone ................................................................... 10.0 g
Peptonized Milk ....................................................... 10.0 g
Agar ......................................................................... 11.0 g


Difcoâ„¢ Tomato Juice Agar Special
Approximate Formula* Per Liter
Tomato Juice (from 400 mL) ..................................... 20.0 g
Peptone ................................................................... 10.0 g
Peptonized Milk ....................................................... 10.0 g
Agar ......................................................................... 20.0 g


Difcoâ„¢ Tomato Juice Broth
Approximate Formula* Per Liter
Tomato Juice (from 400 mL) ..................................... 20.0 g
Yeast Extract ............................................................ 10.0 g
Dextrose ................................................................... 10.0 g
Dipotassium Phosphate .............................................. 0.5 g
Monopotassium Phosphate ........................................ 0.5 g
Magnesium Sulfate .................................................... 0.1 g
Sodium Chloride ........................................................ 0.01 g
Ferrous Sulfate ........................................................... 0.01 g
Manganese Sulfate .................................................... 0.01 g
*Adjusted and